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By substituting helix-favoring alanine residues at these positions, researchers enhanced the amphiphilic -helical conformation critical for GHRH receptor binding

Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation

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