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This can be done using stressful situations or by injecting lipopolysaccharide, an endotoxin from bacteria.[ref] Animals bred not to produce IL-6 are resistant to despair behavior usually induced by stress.[ref] Genetic studies in humans also link higher levels of IL-6, stressful life events, and an increased risk of depression.[ref] Interleukin-6 is one of the major cytokines released by immune cells in response to a pathogen or toxin
J.Med.Sci.(Pakistan) 2006;6(5):800-805
In one study of 53 postmenopausal women, participants abstained from alcohol for 8 weeks, then drank one alcoholic beverage per night for 8 weeks, then drank two alcoholic beverages per night for 8 weeks
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