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The mixture of hA3A-eBE3-Y130F mRNA (100 ng/L) and Trim37- sgRNA-stop (100 ng/L) was diluted in RNase-free water, centrifuged at 13,400 g for 10 min at 4 C, and injected into the cytoplasm of zygotes ( mTmG Blimp1-T2A-Cre or B6D2F1 Nanog-EGFP ) using a micromanipulator (Olympus, Tokyo, Japan) and a FemtoJet microinjector (Eppendorf)

ASNS, asparagine synthetase

No invasive techniques are used, and no anesthesia is required

Figure 5: The technological process of extraction of glutathione from yeast cell Uses 1.Reduced glutathione is a kind of small molecular peptide, a large number of peptides in living organisms, especially in liver cells, protect the liver cell membrane, promote the role of liver enzyme activity, and with a number of toxic chemicals in combination with play the role of detoxification.To the drug poisoning, alcoholism and other causes of liver injury, disease such as cirrhosis of the liver have good curative effect

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4 weeks 1 vial 28 daily draws fit inside one vial at 22.5 units/day