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To address these limitations, we developed a single-fluorophore cpEGFP-based tension indicator that reports force through deformation of the -barrel structure rather than changes in donoracceptor energy transfer

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The velocity of such a ping-pong reaction with infinite true k cat and K m values can be described by the equation [ E ]/ V = 1 /[GSSCys]+ 2 /[GSH], wherein the Dalziel coefficients 1 and 2 are the slopes of secondary plots for k cat app (GSH) and k cat app (GSSCys) , respectively 39 (Supplementary Table 1)