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doi: 10.1016/j.tifs.2024.104847 133

USA 107 , 87888793 (2010)

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Results Synthesis and characterization of EGCG-Zn nanocomplexes In this study, EGCG-Zn nanocomplexes were first synthesized through coordination between Zn(II) ions and the phenolic hydroxyl groups of EGCG, forming stable nanoscale complexes (Fig

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After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader