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The supernatant of tissue was evaporated under a stream of nitrogen at 40 C, reconstituted in 100 L acetonitrile/ultrapure water (90/10) with 0.1% formic acid, and transferred to a 2 mL tube with a 0.20 m centrifugal filter
Remember, while these tips are based on available research, individual results may vary, and professional medical advice should always be sought when needed.
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