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Induction of these chaperones represents an adaptive stress response that protects cardiomyocytes by stabilizing the contractile apparatus and preserving sarcomeric organization during ischemic injury

Cells were dehydrated with an ethanol series (20%, 50%, 70%, 90% and 100%) on ice for 1 min each and then 100% dry ethanol at room temperature three times for 1 min each, infiltrated with 50:50 dry ethanol:Durcupan ACM resin (Sigma-Aldrich, 44610) for 30 min and then four changes of Durcupan at 1 h each and finally embedded in a vacuum oven at 60 C for 72 h

Gentle stretches for the wrist flexors and extensors should be performed multiple times daily, especially before and after activities

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