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They quit at the peak

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Cysteine, the central amino acid in glutathione, contains a carbon atom that is bonded to a sulfur-hydrogen functional group

Jones et al ., 2018), and the use of the cleavable BG-Lumi4-Tb to monitor GLP-1R recycling in combination with a fluorescent antagonist ligand was recently described by our group (Pickford et al ., 2020)

This is because glutathione takes time to accumulate at the cellular level, where it begins neutralising free radicals, reducing oxidative stress, and gradually suppressing melanin synthesis