The sonicated lysate was centrifuged and diluted using 10 volumes of cold ChIPdilution buffer (0.01% SDS, 1.1% Triton X100, 1.2 mM EDTA, 16.7 mM TrisHCl, pH 8, 167 mM NaCl)
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True recovery and symptom management require a holistic approach
Thats why when a scientist discovers a particular protein thats important in a cellular process or disease, one of the first things they will do is examine its molecular interactions
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Evidence suggests that this effect is not related solely to weight reduction and better glycaemic control