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While these mechanisms are well-documented in general skin-brightening studies, the application for those with localized depigmentation is more complex

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[4] The amino acid sequence is Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val

Isopropyl -d-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM and the culture was further incubated at 18 C overnight

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