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Monolayers were infected with either Ad-GFP or Ad-Cre (100 MOI) and efficiency of the IR deletion was verified by measuring IR mRNA and protein expression 48 h after infection (Figure 3a and 3b)

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Protein abundance values were used for further statistical analysis

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Dimerization of substrate adaptors can facilitate cullin-mediated ubiquitylation of proteins by a tethering mechanism: a two-site interaction model for the Nrf2-Keap1 complex

45 Thus, since the 1990s, in vitro-synthesized mRNA has been increasingly applied for protein replacement in preventive and therapeutic vaccines