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Loren Pickart and colleagues isolated a short peptide from human plasma that extended the lifespan of cultured liver cells. They identified its sequenceglycyl-histidyl-lysine (GHK)and discovered that when bound to copper ions, it formed GHK-Cu, a complex with strong biological activity in cell growth and tissue repair

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Freshly purified proteins were subsequently analysed in steady-state kinetic measurements using GSSCys and HEDS as alternative disulfide substrates

Advances in the application of biosynthesis and metabolic engineering of flavonoids in plants