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rapid isolation of inhibitor-free
Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 89
competent cells are highly efficient (> 108 transformants/ µg pUC19) and can be used for cloning
and other enzymatic procedures
3' context
Methylated/Non-methylated Control DNA & Primer Set Size:1 Set rapid isolation of inhibitor-freeThe methylated Non methylated Control DNA is a mixture of fully methylated pUC19 (pUC19m) and nonmethylated pUC19 (pUC19) DNA at a 1: 4 ratio. The Control DNA and Control Primers provided with it can be used for monitoring the different steps of the methylated DNA IP procedure. The pUC19m DNA contains a mutation to create a novel Nco I restriction enzyme site. Since the methylated DNA (pUC19m) contains a Nco I restriction site (the non methylated
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